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Medion Diagnostics giemsa staining diff quik fix
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Giemsa Staining Diff Quik Fix, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
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Dade Behring diff-quik fixative/stain set
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Diff Quik Fixative/Stain Set, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diff-quik fixative/stain set/product/Dade Behring
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diff-quik fixative/stain set - by Bioz Stars, 2026-03
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Dade Behring diff-quik® fixative and stain set
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Diff Quik® Fixative And Stain Set, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diff-quik® fixative and stain set/product/Dade Behring
Average 90 stars, based on 1 article reviews
diff-quik® fixative and stain set - by Bioz Stars, 2026-03
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Dade Behring diff-quik fixative
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Diff Quik Fixative, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/diff-quik fixative/product/Dade Behring
Average 90 stars, based on 1 article reviews
diff-quik fixative - by Bioz Stars, 2026-03
90/100 stars
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Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Journal: Frontiers in Immunology

Article Title: Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils

doi: 10.3389/fimmu.2021.636954

Figure Lengend Snippet: Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Article Snippet: The preparations contained >99% granulocytes, of which >96% were neutrophils and 1%–4% were eosinophils, as determined by Giemsa staining (Diff Quik ® Fix, Medion Diagnostics, Berlin, Germany) of cytocentrifuged (Shandon) samples ( ).

Techniques: Activation Assay, Flow Cytometry, Staining, Fluorescence, Microscopy